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Item #:
84061248
Citrate Buffer, 1M, pH 6.0, 250 mL
Item #:
84061248
BSD 71
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What Stands Out
Optimal pH Control
Ensures reliable results in experiments by maintaining a stable pH of 6.0, critical for biochemical and molecular applications.
High Concentration
This 1M concentration offers enhanced buffer capacity, effectively stabilizing pH during reactions and providing robust support for various laboratory applications.
Convenient Volume
With a 250 mL volume, this buffer provides ample supply for multiple experiments, reducing the need for frequent reordering and optimizing lab efficiency.
Product Details
| Item Weight | 0.55 lbs (250 grams) |
Who Should Buy?
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Biochemical Research
Ideal for laboratories conducting enzyme assays and other biochemical reactions requiring a stable pH environment.
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Molecular Biology
Beneficial for molecular biologists needing a reliable buffer for DNA/RNA purification and manipulation.
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Pharmaceutical Applications
Useful in the pharmaceutical industry for drug formulation and stability testing that requires specific pH conditions.
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High-Temperature Experiments
Not suitable for experiments requiring high temperatures, as buffer stability may be compromised.
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Basic pH Applications
Not ideal for applications requiring buffers with a pH above 7.0, limiting its use in alkaline processes.
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Non-Aqueous Solutions
Inappropriate for use in non-aqueous solvent systems where citrate buffer will not function effectively.
Product Description
Citrate Buffer, 1M, pH 6.0, 250 mL
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Customer Questions & Answers
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Question:
What is Citrate Buffer, 1M, pH 6.0 used for?
Answer: Citrate Buffer, 1M, pH 6.0, is primarily utilized in biochemical applications, particularly in molecular biology and biochemistry. It acts as a stabilizing agent that maintains pH conditions during various experiments, including enzyme reactions and protein assays. This buffer is essential for ensuring consistent results in DNA and RNA studies, as many assays require a specific pH for optimal performance. -
Question:
How do you prepare Citrate Buffer, 1M, pH 6.0?
Answer: To prepare Citrate Buffer, 1M, pH 6.0, mix 1M citric acid and sodium citrate at a ratio that achieves the desired pH level. Typically, this involves combining 0.1M citric acid and 0.1M sodium citrate in appropriate proportions and adjusting the pH using a pH meter. Proper preparation is crucial for obtaining accurate results in your experiments, particularly when testing acids or bases, or calibrating pH-sensitive equipment. -
Question:
Can I use Citrate Buffer for cell culture applications?
Answer: Citrate Buffer, 1M, pH 6.0 is not typically used directly in cell culture applications, but it can be beneficial for specific experimental setups that require a stable pH environment. For example, researchers might use it for washing cells or preparing reagents where pH stabilization is crucial. Always consider the specific requirements of your cell line before application, as pH sensitivity varies across different cell types. -
Question:
Is Citrate Buffer, 1M, pH 6.0 suitable for protein analyses?
Answer: Yes, Citrate Buffer, 1M, pH 6.0 is ideal for protein analyses, particularly in assays involving enzyme activity and protein stability. Many proteins function optimally at a pH around 6.0, making this buffer perfect for maintaining conditions that are conducive to accurate measurements. This buffer helps minimize fluctuations that could impact enzyme kinetics or structural integrity during analysis. -
Question:
What are the storage requirements for Citrate Buffer, 1M, pH 6.0?
Answer: Citrate Buffer, 1M, pH 6.0 should be stored in a cool, dry place, away from direct sunlight, to maintain its stability. Ideally, it should be stored at room temperature, in a tightly sealed container to prevent contamination. Proper storage is essential because it ensures the buffer remains effective for its intended applications, which may involve metabolic or chemical reactions sensitive to environmental changes. -
Question:
Can Citrate Buffer be used in clinical laboratories?
Answer: Yes, Citrate Buffer, 1M, pH 6.0 finds application in clinical laboratories, particularly in diagnostic assays where maintaining a specific pH is vital for accuracy. It is often used in coagulation studies and sample preparations where pH can affect the behavior of various biomolecules. Its reliable performance makes it a staple in routine laboratory practices. -
Question:
What precautions should I take when using Citrate Buffer?
Answer: When using Citrate Buffer, 1M, pH 6.0, it is essential to wear appropriate PPE, including gloves and goggles, to protect against potential splashes. Additionally, ensure that your workspace is clean and free from contaminants that could affect experimental results. Practicing good laboratory hygiene is critical to maintaining the integrity of your experiments and avoiding cross-contamination. -
Question:
Is Citrate Buffer compatible with other buffer systems?
Answer: Citrate Buffer, 1M, pH 6.0 can be compatible with certain other buffer systems depending on the specific requirements of your application. For example, it may be mixed with phosphate buffers in certain scenarios to achieve a desired pH or ionic strength. However, care must be taken, as combining buffers can lead to unpredictable results; it’s pivotal to test compatibility for your specific experimental conditions. -
Question:
What is the role of pH in experiments using Citrate Buffer?
Answer: In experiments involving Citrate Buffer, pH plays a crucial role in influencing molecular interactions and enzyme activity. Many biochemical processes are pH-dependent; thus, maintaining a stable pH at 6.0 is essential for achieving reproducibility in experimental results. It's particularly relevant in enzyme assays where deviations from optimal pH can lead to altered reaction rates or product formation. -
Question:
Where can I buy Citrate Buffer, 1M, pH 6.0, 250 mL in The Bahamas?
Answer: You can purchase Citrate Buffer, 1M, pH 6.0, 250 mL from Ubuy in The Bahamas. Ubuy provides a reliable platform for sourcing quality laboratory reagents, including this essential buffer. By shopping there, you can ensure that you are getting a product that meets high standards for your experimental and research needs.
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Pros
- Highly effective pH stabilizer
- Easy to use and measure
- Compatible with various applications
- Reliable performance across experiments
- Well-packaged for safe handling
Cons
- Occasional sediment formation
Product Price History
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Order now and get it around Sunday, July 05
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Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Features & Benefits
- Comprehensive range of biological buffers
- Buffers important for protein research and molecular biology
- Manufactured under stringent Good Manufacturing Practices
- Citrate buffer's versatile applications in RNA isolation and immunofluorescent staining
- Popular choice for biological research and clinical applications
- For research use only. Not for human or animal clinical/therapeutic use