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- Hanks Balanced Salt Solution HBSS with Calciu...
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Item #:
80788535
Hanks Balanced Salt Solution HBSS with Calcium & Magnesium
Item #:
80788535
BSD 29
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Hygia’s balanced salt solutions are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU.
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What Stands Out
Balanced Formulation
HBSS provides an optimal pH and ionic balance, essential for maintaining cell viability during experiments and ensuring consistent results in cell culture applications.
Essential Ions
The inclusion of calcium and magnesium supports critical cellular processes, enhancing cell adhesion and growth, making it ideal for various biological studies.
Versatile Applications
Designed for use in diverse research fields, HBSS is suitable for cell culture, tissue preservation, and in vitro experimentation, addressing the needs of scientists across multiple disciplines.
Product Details
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Cell Culture
Ideal for maintaining and culturing various cell types due to its balanced ionic composition and nutrients.
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Biomedical Research
Essential for experiments requiring stable osmotic pressure and appropriate pH for cellular functions in research settings.
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Tissue Preservation
Suitable for preserving tissues during transportation or experimental procedures, ensuring optimal conditions for viability.
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Non-Cell Applications
Not suitable for applications unrelated to cell culture, as it may not meet specific requirements of other protocols.
Product Description
Hanks Balanced Salt Solution HBSS with Calcium & Magnesium
About This Item
Introducing Hanks' Balanced Salt Solution HBSS with Calcium & Magnesium, the ideal solution for all your cell culture needs. This high-quality solution provides the perfect balance of essential salts and minerals to support cell growth and maintenance. Our Hanks' Balanced Salt Solution is designed to create an optimal physiological environment for cells, ensuring their viability and functionality. With added calcium and magnesium, this solution further enhances cell performance and stability, making it an essential tool for cell culture research and applications. At our online Ubuy store, you can purchase this top-grade Hanks' Balanced Salt Solution with ease and confidence.
We are committed to providing you with the best products for your scientific research and cell culture requirements. Order now and experience fast shipping and free delivery, making it convenient and cost-effective to stock up on this essential solution for your laboratory. Trust in the quality and reliability of our Hanks' Balanced Salt Solution HBSS with Calcium & Magnesium, and take your cell culture experiments to the next level. Purchase now and elevate your cell culture research with the best tools available.
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Customer Questions & Answers
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Question:
What is Hanks’ Balanced Salt Solution (HBSS) used for?
Answer: Hanks’ Balanced Salt Solution is primarily used in biological and biochemical research. It serves as a nutrient medium to support the growth and maintenance of cells, tissues, and organs in culture. The inclusion of calcium and magnesium is essential for promoting cell adhesion and functionality, crucial for experiments involving cell viability and proliferation. For example, HBSS can be employed in tissue engineering studies or when isolating primary cells from tissues. -
Question:
What are the key components of Hanks’ Balanced Salt Solution?
Answer: Hanks’ Balanced Salt Solution consists of a mix of salts, glucose, and buffering agents that help maintain the physiological pH and osmotic balance in cell cultures. The presence of calcium and magnesium is significant as they play essential roles in various cellular processes, including enzyme activity and signal transduction. This makes it ideal for applications like cell dissociation or when preparing tissues for experiments, ensuring optimal cellular function. -
Question:
Is Hanks’ Balanced Salt Solution suitable for all cell types?
Answer: While Hanks’ Balanced Salt Solution is generally suitable for a wide variety of cell types, it may not be ideal for certain specialized cell lines that require specific nutrients or growth factors. The nutrient composition supports many mammalian cells, making it excellent for studies involving fibroblasts, epithelial cells, and some cancer cell lines. Researchers should evaluate their specific cell requirements to determine the suitability of HBSS in their protocols. -
Question:
How should Hanks’ Balanced Salt Solution be stored?
Answer: Hanks’ Balanced Salt Solution should be stored in a cool, dry place, preferably at 2 to 8 degrees Celsius to maintain its stability and effectiveness. Proper storage ensures that the physiological properties are preserved, allowing researchers to rely on its performance during experiments. It’s advisable to avoid repeated freeze-thaw cycles, which can deteriorate the solution. Storing it correctly can extend its usability for critical experiments. -
Question:
Can Hanks’ Balanced Salt Solution be used for washing cells?
Answer: Yes, Hanks’ Balanced Salt Solution is frequently utilized for washing cells prior to experimentation or after trypsinization. Its isotonic properties help to remove excess culture media and debris without damaging the cells. This is essential when preparing samples for analysis or prior to seeding cells into assays, ensuring that only the desired cells are subjected to further treatments or assays, ultimately improving experimental accuracy. -
Question:
What is the importance of calcium and magnesium in HBSS?
Answer: Calcium and magnesium are vital components in Hanks’ Balanced Salt Solution that support critical cellular functions such as adhesion, signaling, and the maintenance of membrane integrity. These divalent cations play key roles in stabilizing cellular environments, particularly for adherent cells. In experiments such as those involving cell migration or growth factor responses, the presence of these ions can significantly influence the outcomes by enhancing cellular interactions. -
Question:
How do you prepare Hanks’ Balanced Salt Solution for use?
Answer: To prepare Hanks’ Balanced Salt Solution for laboratory use, it is important to correctly dissolve the powdered form in sterile distilled water or buffer and adjust the pH to physiological levels, typically around 7.2 to 7.4. This ensures that the solution provides an optimal environment for cells. Always work in aseptic conditions to maintain sterility, and consider filtering it through a 0.22-micron filter before usage to ensure it is free of contaminants. -
Question:
What are some common applications of Hanks’ Balanced Salt Solution in labs?
Answer: Hanks’ Balanced Salt Solution is widely used in various laboratory applications, including cell culture, enzyme assays, and tissue preservation. It serves as a reliable medium for maintaining cell viability during experimentation and is also applied in protocols for cell washing and dilutions. Typical scenarios include serum-free cell culture setups where maintaining osmotic balance is crucial or during the preparation of samples for flow cytometry and immunostaining. -
Question:
Can I use Hanks’ Balanced Salt Solution for in vivo applications?
Answer: Hanks’ Balanced Salt Solution is primarily designed for in vitro applications and is not suitable for in vivo use. This is because the solution lacks essential nutrients and growth factors required by living organisms. Using HBSS in in vivo scenarios might lead to adverse effects or insufficient support for tissue health. Researchers should explore other formulations created specifically for in vivo conditions when working with live subjects. -
Question:
Where can I buy Hanks’ Balanced Salt Solution HBSS with Calcium & Magnesium in The Bahamas?
Answer: You can buy Hanks’ Balanced Salt Solution HBSS with Calcium & Magnesium through Ubuy. They offer a wide selection of laboratory supplies, ensuring you find quality products tailored to your research needs. Ubuy provides a straightforward shopping experience, making it easier to access essential lab materials conveniently.
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Features & Benefits
- Manufactured at cGMP-compliant facility
- Filtered through final 0.1 μm sterile filtration
- Aseptic filled in a Hundred-stage FFU
- Undergoes sterility test, pH, osmolality and endotoxin checks
- Suitable for various cell culture applications
- Available in 500 mL size
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